Journal: Journal of Nanobiotechnology

Article Title: Cerium oxide nanoparticles-carrying human umbilical cord mesenchymal stem cells counteract oxidative damage and facilitate tendon regeneration

doi: 10.1186/s12951-023-02125-5

Figure Lengend Snippet: Characterization of hUCMSCs. A Primary hUCMSCs were spindle-shaped under light microscopy. B Flow cytometry analysis showed that 98.5% of cells expressed CD29, 97.4% expressed CD44, 98.6% of cells expressed CD90, and 98.1% of cells expressed CD105. Meanwhile, only 0.48% and 0.28% expressed CD45 and CD34. C Lipid droplets were stained by oil red O after adipogenic differentiation induction of hUCMSCs. Mineralized nodules were stained by Alizarin Red after osteogenic differentiation induction of hUCMSCs. Acid mucopolysaccharide was stained by Alcian Blue after chondrogenic differentiation induction of hUCMSCs

Article Snippet: Briefly, 10 7 hUCMSCs were subjected to analysis for surface markers CD34 (E-AB-F1143D, Elabscience), CD44 (E-AB-F1100D, Elabscience), CD45 (E-AB-F1137D, Elabscience), CD29 (E-AB-F1049D, Elabscience), CD90 (E-AB-F1167D, Elabscience), and CD105 (E-AB-F1310D, Elabscience) by flow cytometry following the manufacturer’s protocol.

Techniques: Light Microscopy, Flow Cytometry, Staining

Journal: Molecular Therapy Nucleic Acids

Article Title: Ligand-modified rAAV6 vectors with nanoblades allow high level gene knock-in in HSPCs without compromising cell survival

doi: 10.1016/j.omtn.2025.102495

Figure Lengend Snippet: Figure 5. Coupling of rAAV6 with low amounts of mannose allowed high-level nanoblade-mediated knockin CD34+ cells without cell death induction (A) 8E4, 3E5, or 8E5 mannose sugars were incubated for 4 h at RT with 10E12 vg of rAAV6 vectors encoding for the donor template. 10E9 vg rAAV6 vector per condition were analyzed in dot blot using the ADK1 antibody for rAAV6 that recognizes the intact assembled capsid or conca- navalin A, which detected mannose bound to the capsid. (B) 5E8 vg of rAAV6 conjugated with indicated quantities of mannose were separated on a gel, followed by silver staining to reveal the intact capsids. (C and D) CD34+

Article Snippet: Purity of the selected CD34+ cell fraction was evaluated by fluorescence-activated cell sorting (FACS) analysis (FACSCanto, BD) with APC-conjugated anti-CD34 antibody (Miltenyi Biotech).

Techniques: Knock-In, Incubation, Plasmid Preparation, Dot Blot, Silver Staining

Journal: Neural Regeneration Research

Article Title: A vascular endothelial growth factor–loaded chitosanhyaluronic acid hydrogel scaffold enhances the therapeutic effect of adipose-derived stem cells in the context of stroke

doi: 10.4103/nrr.nrr-d-24-00129

Figure Lengend Snippet: Figure 1 ｜ADSC culture and identity verification, and the effect of VEGF on ADSCs. (A) Representative images of ADSCs on days 3 (i) and 5 (ii) of primary culture, as well as third-passage ADSCs (iii). On day 3, the cells were spindle-shaped, isolated, scattered, and adherent. By day 5, the cells had proliferated rapidly, and the cell colonies had expanded. The morphology of the third-passage cells was uniform, displaying a characteristic fish-like, fibrous, and swirling arrangement. Scale bar: 100 μm. (B) Flow cytometric analysis of CD90, CD29, CD34, and CD45 expression on the cell surface of third-passage ADSCs. (C) CCK-8 analysis of the viability of ADSCs treated with different concentrations of VEGF. Data are expressed as mean ± SEM (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, vs. Cont group (one-way analysis of variance with Bonferroni’s post hoc test). (D) Enzyme-linked immunosorbent assay analysis of VEGF, b-FGF, TGF-β, BDNF, and IGF-1 concentration by ADSC cells treated with VEGF (50 ng/mL) or left untreated. Data are expressed as mean ± SEM (n = 12). **P < 0.01, ***P < 0.001, vs. VEGF- group (one-way analysis of variance with Bonferroni’s post hoc test [C] or unpaired t-test [D]). ADSCs: Adipose-derived mesenchymal stem cells; BDNF: brain-derived neurotrophic factor; b-FGF: basic fibroblast growth factor; Cont: control; IGF- 1: insulin-like growth factor-1; TGF-β: transforming growth factor-β; VEGF: vascular endothelial growth factor.

Article Snippet: The following fluorochrome-labeled antibodies were utilized: APC-cy7-CD45 (BD Biosciences, San Jose, CA, USA, Cat# 561586, RRID: AB_10896305), PE-CD34 (RayBiotech, Norcross, GA, USA, Cat# 188-10041-PE, RRID: AB_2315728), FITC-CD90 (BD Biosciences, Cat# 561973, RRID: AB_395588), and BV421-CD29 (BD Biosciences, Cat# 564131, RRID: AB_2738613).

Techniques: Isolation, Expressing, CCK-8 Assay, Enzyme-linked Immunosorbent Assay, Concentration Assay, Derivative Assay, Control

Journal: Cells

Article Title: IMG-A1: A Novel Immortalized Granulosa Cell Line for Investigating FSH-Dependent Folliculogenesis and Ovarian Pathophysiology

doi: 10.3390/cells14241940

Figure Lengend Snippet: Basic Characterization of the IMG-A1 Granulosa Cell Line. ( a , b ) Phase-contrast micrographs of the IMG-A1 cell culture. ( c ) Staining for senescence-associated β-galactosidase activity in passage 18 cells. ( d – f ) Immunocytochemical staining for the proliferation marker Ki67, showing a phase-contrast image ( d ), Ki67 immunofluorescence ( e ), and a merged image with DAPI nuclear counterstain ( f ). ( g , h ) Representative metaphase spreads showing a normal diploid karyotype (40 chromosomes) ( g ) and a near-tetraploid karyotype (78 chromosomes) from the IMG-A1 line ( h ). ( i – k ) Ploidy analysis by flow cytometry. Control blood cells ( i ) and primary granulosa cells ( j ) show characteristic diploid (2n) and tetraploid (4n, G2/M phase) peaks. The IMG-A1 culture ( k ) displays a predominantly near-tetraploid and near-octaploid cell population. ( l ) Flow cytometry analysis confirming the homogeneity of the IMG-A1 culture (passages 10 and 40) based on staining for the stromal marker CD29 and the absence of hematopoietic markers CD45, CD34, and the fibroblast marker CD90. Quadrant gates were set based on unstained controls. Scale bars = 100 µm.

Article Snippet: PE CD34 Rat mAb [RAM34] , Elabscience Biotechnology Co., Ltd., Wuhan, China , E-AB-F1284D.

Techniques: Cell Culture, Staining, Activity Assay, Marker, Immunofluorescence, Flow Cytometry, Control

Journal: Cell systems

Article Title: Analysis of Body-wide Unfractionated Tissue Data to Identify a Core Human Endothelial Transcriptome.

doi: 10.1016/j.cels.2016.08.001

Figure Lengend Snippet: Figure 1. CLEC14A, vWF, and CD34 Tran- script Quantities In Vivo Reflect the Degree of Tissue Vascularization (A) Mean FPKM values for c-type lectin domain family 14, member A (CLEC14A), von Willebrand factor (vWF), and CD34 (CD34) transcripts in bone marrow, pancreas, ovary, tonsil, salivary gland, appendix, spleen, thyroid gland, gallbladder, uri- nary bladder, heart muscle, and lung; n = 2–5 in- dividual samples/organ (see Table S1). Data are mean ± SEM. Corresponding IHC images stained with primary antibodies against CLEC14A, vWF, and CD34 protein are shown on tissue sections from ovary, appendix, gall bladder, and lung. (B) Scatterplots showing correlations between mean CLEC14A, vWF, and CD34 FPKM values and the estimated mean EC percentage in the sequenced sample, determined by histological examination prior to processing. Tissue type rep- resented by each symbol corresponds to that indicated on the x axis of (A). Pearson correlation and corresponding p values are shown in the top left of each scatterplot. See also Figure S1A. Scale bars, 100 mm.

Article Snippet: Primary antibody against CLEC14A (Atlas Antibodies HPA039468), VWF (Atlas Antibodies HPA001815), CD34 (Atlas Antibodies HPA036722), HSPA12B (Atlas Antibodies HPA013659), ENG (Novocastra NCL-CD105), ESM1 (Atlas Antibodies HPA036660), PTPRC (Atlas Antibodies HPA000440), ITGA2B (Atlas AntibodiesHPA031168),MYH11 (Atlas AntibodiesHPA0145359), EDH2 (AtlasAntibodiesHPA049890), LIMS2 (AtlasAntibodies HPA058340), FAM110D (Atlas Antibodies HPA013664), KANK3 (Atlas Antibodies HPA051153) or GIPC3 (Atlas Antibodies HPA061258) and a dextran polymer visualization system (UltraVision LP HRP polymer, Lab Vision) were incubated for 30 min each at room temperature and slides were developed for 10 min using Diaminobenzidine (Lab Vision) as the chromogen.

Techniques: In Vivo, Staining

Journal: Cell systems

Article Title: Analysis of Body-wide Unfractionated Tissue Data to Identify a Core Human Endothelial Transcriptome.

doi: 10.1016/j.cels.2016.08.001

Figure Lengend Snippet: Figure 2. Correlation Values between the Reference Endothelial Cell Transcripts CLEC14A, vWF, CD34 and Proteins Des- cribed as EC Enriched in the Literature (A) RNA-seq data from 124 individual samples from 32 different human tissue types were used to generate Spearman pair wise correlation values between the EC reference transcripts CLEC14A, vWF, and CD34 and transcripts reported in the literature as EC enriched. (B) IHC images of salivary gland, gallbladder, and lung tissue stained for proteins encoded by HSPA12B, PECAM1, ENG, ESM1, LIPG, and EDF1. Corresponding scatterplots (right) show the correlation between mean FPKM values and mean EC percentage in selected sequenced tissue samples. Tissue type represented by each symbol corresponds to that indicated on the x axis of Figure 1A. Pearson correlations and correspond- ing p values are shown for each scatterplot. Scale bars, 50 mm.

Article Snippet: Primary antibody against CLEC14A (Atlas Antibodies HPA039468), VWF (Atlas Antibodies HPA001815), CD34 (Atlas Antibodies HPA036722), HSPA12B (Atlas Antibodies HPA013659), ENG (Novocastra NCL-CD105), ESM1 (Atlas Antibodies HPA036660), PTPRC (Atlas Antibodies HPA000440), ITGA2B (Atlas AntibodiesHPA031168),MYH11 (Atlas AntibodiesHPA0145359), EDH2 (AtlasAntibodiesHPA049890), LIMS2 (AtlasAntibodies HPA058340), FAM110D (Atlas Antibodies HPA013664), KANK3 (Atlas Antibodies HPA051153) or GIPC3 (Atlas Antibodies HPA061258) and a dextran polymer visualization system (UltraVision LP HRP polymer, Lab Vision) were incubated for 30 min each at room temperature and slides were developed for 10 min using Diaminobenzidine (Lab Vision) as the chromogen.

Techniques: RNA Sequencing, Staining

Journal: Cell systems

Article Title: Analysis of Body-wide Unfractionated Tissue Data to Identify a Core Human Endothelial Transcriptome.

doi: 10.1016/j.cels.2016.08.001

Figure Lengend Snippet: Figure 3. Summary of Endothelial Cell Reference Transcript Correlation Analysis Data RNA-seq data from 124 individual samples from 32 different human tissue types were used to generate pairwise correlation values between the EC reference transcripts CLEC14A, vWF, and CD34 and the other 20,073 detectable protein-coding genes. (A) 234 transcripts were identified as EC enriched and categorized as known (previously reported as EC expressed), unknown (not reported as EC expressed), or uncharacterized. The ten most highly correlated in each category are displayed (p < 0.001 in all cases). (B) Scatterplots showing the correlation between mean FPKM values for selected genes from each category and the mean EC percentage in the sequenced tissue sample, determined by histological examination prior to processing. Tissue type represented by each symbol corresponds to that indicated on the x axis of Figure 1A. Pearson correlation and corresponding p values are shown in the top left of each scatterplot. See also Table S3, tab 1.

Article Snippet: Primary antibody against CLEC14A (Atlas Antibodies HPA039468), VWF (Atlas Antibodies HPA001815), CD34 (Atlas Antibodies HPA036722), HSPA12B (Atlas Antibodies HPA013659), ENG (Novocastra NCL-CD105), ESM1 (Atlas Antibodies HPA036660), PTPRC (Atlas Antibodies HPA000440), ITGA2B (Atlas AntibodiesHPA031168),MYH11 (Atlas AntibodiesHPA0145359), EDH2 (AtlasAntibodiesHPA049890), LIMS2 (AtlasAntibodies HPA058340), FAM110D (Atlas Antibodies HPA013664), KANK3 (Atlas Antibodies HPA051153) or GIPC3 (Atlas Antibodies HPA061258) and a dextran polymer visualization system (UltraVision LP HRP polymer, Lab Vision) were incubated for 30 min each at room temperature and slides were developed for 10 min using Diaminobenzidine (Lab Vision) as the chromogen.

Techniques: RNA Sequencing

Journal: Scientific Reports

Article Title: Analysis of the spatial-temporal distribution and functional morphology of telocytes in goat testes

doi: 10.1038/s41598-026-36639-3

Figure Lengend Snippet: DIF of CD34/α-SMA in 1-month-old goat testis. ( A-C ) DIF of CD34/α-SMA in 1-month-old goat testis; ( D-F ) DIF of CD34/α-SMA in blood vessels of 1-month-old goat testis; CD34 (red); α-SMA (green); DAPI (blue); CD34 + cells (white triangular arrows); ST. Seminiferous tubule; BV. Blood vessels. Scale bar = ( A-C ):10 μm; ( D-F ): 20 μm.

Article Snippet: The antibody pairs were as follows: CD34 (1:100, BA3414, Boster Biological Technology Co., Ltd)/α-SMA (1:100, BM0002, Boster Biological Technology Co., Ltd), CD34 (1:100)/Vimentin (1:100, BM0135, Boster Biological Technology Co., Ltd), α-SMA (1:100)/PDGFR-α (1:100, 3174 S, CellSignaling Technology).

Techniques:

Journal: Scientific Reports

Article Title: Analysis of the spatial-temporal distribution and functional morphology of telocytes in goat testes

doi: 10.1038/s41598-026-36639-3

Figure Lengend Snippet: DIF of CD34/α-SMA in 2-month-old goat testis. ( A-C ) DIF of CD34/α-SMA in 2-month-old goat testis; ( D-F ) DIF of CD34/α-SMA in blood vessels of 2-month-old goat testis; CD34 (red); α-SMA (green); DAPI (blue); CD34 + cells (white triangular arrows); ST. Seminiferous tubule; BV. Blood vessels. Scale bar = ( A-C ): 20 μm; ( D-F ): 10 μm.

Article Snippet: The antibody pairs were as follows: CD34 (1:100, BA3414, Boster Biological Technology Co., Ltd)/α-SMA (1:100, BM0002, Boster Biological Technology Co., Ltd), CD34 (1:100)/Vimentin (1:100, BM0135, Boster Biological Technology Co., Ltd), α-SMA (1:100)/PDGFR-α (1:100, 3174 S, CellSignaling Technology).

Techniques:

Journal: Scientific Reports

Article Title: Analysis of the spatial-temporal distribution and functional morphology of telocytes in goat testes

doi: 10.1038/s41598-026-36639-3

Figure Lengend Snippet: DIF of CD34/α-SMA in 12-month-old goat testis. ( A-I ) DIF of CD34/α-SMA in 12-month-old goat testis; ( J-L ) DIF of CD34/α-SMA in blood vessels of 12-month-old goat testis; CD34 (red); α-SMA (green); DAPI (blue); ST. Seminiferous tubule; BV. Blood vessels. Scale bar = ( A-F, J-L ): 10 μm; ( G-I ): 20 μm.

Article Snippet: The antibody pairs were as follows: CD34 (1:100, BA3414, Boster Biological Technology Co., Ltd)/α-SMA (1:100, BM0002, Boster Biological Technology Co., Ltd), CD34 (1:100)/Vimentin (1:100, BM0135, Boster Biological Technology Co., Ltd), α-SMA (1:100)/PDGFR-α (1:100, 3174 S, CellSignaling Technology).

Techniques:

Journal: Scientific Reports

Article Title: Analysis of the spatial-temporal distribution and functional morphology of telocytes in goat testes

doi: 10.1038/s41598-026-36639-3

Figure Lengend Snippet: DIF of CD34/ Vimentin in 1-month-old goat testis. ( A-C ) DIF of CD34/ Vimentin in 1-month-old goat testis. (1-a), (1-b), (1-c) are respectively enlarged images of (1) in ( A ), ( B ), ( C ). (2-a), (2-b) and (2-c) are the enlarged images of (2) in ( A ), ( B ) and ( C ) respectively. CD34 (red); α-SMA (green); DAPI (blue); TC (yellow, indicated by white triangular arrows); ST. Seminiferous tubule; SC. Sertoli cells. Scale bar = ( A, B, C ): 20 μm; (1 - a) - (1 - c), (2- a) - (2 - c): 10 μm.

Article Snippet: The antibody pairs were as follows: CD34 (1:100, BA3414, Boster Biological Technology Co., Ltd)/α-SMA (1:100, BM0002, Boster Biological Technology Co., Ltd), CD34 (1:100)/Vimentin (1:100, BM0135, Boster Biological Technology Co., Ltd), α-SMA (1:100)/PDGFR-α (1:100, 3174 S, CellSignaling Technology).

Techniques:

Journal: Scientific Reports

Article Title: Analysis of the spatial-temporal distribution and functional morphology of telocytes in goat testes

doi: 10.1038/s41598-026-36639-3

Figure Lengend Snippet: DIF of CD34/ Vimentin in 2-month-old goat testis. ( A-C ) DIF of CD34/ Vimentin in 2-month-old goat testis. (a-c) are enlarged views of the boxed areas in ( A-C ). CD34 (red); α-SMA (green); DAPI (blue); TC (yellow, indicated by white triangular arrows); ST. Seminiferous tubule. Scale bar = ( A-C ): 20 μm; (a-c): 10 μm.

Article Snippet: The antibody pairs were as follows: CD34 (1:100, BA3414, Boster Biological Technology Co., Ltd)/α-SMA (1:100, BM0002, Boster Biological Technology Co., Ltd), CD34 (1:100)/Vimentin (1:100, BM0135, Boster Biological Technology Co., Ltd), α-SMA (1:100)/PDGFR-α (1:100, 3174 S, CellSignaling Technology).

Techniques:

Journal: Scientific Reports

Article Title: Analysis of the spatial-temporal distribution and functional morphology of telocytes in goat testes

doi: 10.1038/s41598-026-36639-3

Figure Lengend Snippet: DIF of CD34/ Vimentin in 12-month-old goat testis. ( A-C ) DIF of CD34/ Vimentin in 12-month-old goat testis. CD34 (red); Vimentin (green); DAPI (blue); ST. Seminiferous tubule; S. sperm; PS. Primary spermatocyte; SC. Sertoli cells; RS. Round sperm cells; White triangular arrows. Peritubular myoid cell nucleus. Scale bar = ( A-C ): 10 μm.

Article Snippet: The antibody pairs were as follows: CD34 (1:100, BA3414, Boster Biological Technology Co., Ltd)/α-SMA (1:100, BM0002, Boster Biological Technology Co., Ltd), CD34 (1:100)/Vimentin (1:100, BM0135, Boster Biological Technology Co., Ltd), α-SMA (1:100)/PDGFR-α (1:100, 3174 S, CellSignaling Technology).

Techniques:

Journal: Scientific Reports

Article Title: Analysis of the spatial-temporal distribution and functional morphology of telocytes in goat testes

doi: 10.1038/s41598-026-36639-3

Figure Lengend Snippet: DIF of CD34/ Vimentin in 12-month-old goat testis. ( A-C ) DIF of CD34/ Vimentin in 12-month-old goat testis. (a-c) is an enlarged view of the white dotted box area of ( A-C ). CD34 (red); Vimentin (green); DAPI (blue); TC (yellow, indicated by white triangular arrows). Scale bar = ( A-C ), (a-c): 20 μm.

Article Snippet: The antibody pairs were as follows: CD34 (1:100, BA3414, Boster Biological Technology Co., Ltd)/α-SMA (1:100, BM0002, Boster Biological Technology Co., Ltd), CD34 (1:100)/Vimentin (1:100, BM0135, Boster Biological Technology Co., Ltd), α-SMA (1:100)/PDGFR-α (1:100, 3174 S, CellSignaling Technology).

Techniques:

Journal: Scientific Reports

Article Title: Analysis of the spatial-temporal distribution and functional morphology of telocytes in goat testes

doi: 10.1038/s41598-026-36639-3

Figure Lengend Snippet: DIF of CD34/ Vimentin in 12-month-old goat testis. ( A-C ) DIF of CD34/ Vimentin in 12-month-old goat testis. (1-a), (1-b), (1-c) are respectively enlarged images of (1) in ( A-C ). (2-a), (2-b), (2-c) are the enlarged images of (2) in ( A-C ). CD34 (red); Vimentin (green); DAPI (blue); TC (yellow); White triangular arrows. Peritubular myoid cell nucleus. Scale bar = ( A-C ): 50 μm; (1-a) - (1-c), (2-a) - (2-c): 10 μm.

Article Snippet: The antibody pairs were as follows: CD34 (1:100, BA3414, Boster Biological Technology Co., Ltd)/α-SMA (1:100, BM0002, Boster Biological Technology Co., Ltd), CD34 (1:100)/Vimentin (1:100, BM0135, Boster Biological Technology Co., Ltd), α-SMA (1:100)/PDGFR-α (1:100, 3174 S, CellSignaling Technology).

Techniques:

Journal: Human cell

Article Title: Effect of psoralen on the regulation of osteogenic differentiation induced by periodontal stem cell-derived exosomes.

doi: 10.1007/s13577-023-00918-2

Figure Lengend Snippet: Fig. 6 A model for the mechanism of osteogenic differentiation of periodontal stem cells regulated by psoralen

Article Snippet: 100 μL cell suspension was added into centrifuge tubes, then mouse anti-human antibodies CD34 (E-AB-F1143C), CD45 (E-AB-F1137C), CD105 (E-AB-F1143D), CD73 (E-AB-F1242D), and CD90 (E-AB-F1167D) (Elabscience, Wuhan, China) (1:50) were added, respectively, PBS was added as the control group, and all cells were incubated at room temperature for 20 min in the dark.

Techniques: